General review on the oxidative folding of small disulfide-rich proteins are available
[Arolas et al, 2006;
Craik 2010].
|
Spider toxins
|
Mature GsMTx4 is able to fold properly
Oxidative folding of mature GsMTx4 yields the correct folded peptide [Ostrow
et al., 2003]. In the absence of GSSG/GSSH, oxidation of the linear precursor
yields a mixture of misfolded forms with incorrectly paired disulfides. After addition
of GSSG/GSSH, the mixture shifted to the correctly folded state. The propeptides
are unnecessary for proper folding. These results are consistent with previous results
on ω-conotoxin MVIIA.
|